NSH 2009 Annual Symposium/Convention

The birth of histologic technique dates back to 1664 when Robert Hooke cut sections of cork with his pen knife and observed them under a microscope. In 1670, Leeuwenhoek made sections from a quill, a bovine optic nerve and the centers of dried flowers using his hand sharpened razor. These events were the beginning of what was to become an important and unique laboratory discipline. Histology is a structural science concerned with the demonstration of cellular morphology, chemical composition and function of normal and abnormal tissue. Many dyes and chemicals are used in histology and it is necessary to know their composition and how they act and react with each other. This knowledge, combined with an understanding of tissue composition, enables the histotechnologist to appropriately treat the tissue of interest. The end result yields a tissue section exhibiting distinct colors, making it possible to distinguish tissue structures through microscopic examination.