Protein-Protein Interactions as Drug Targets 2011

Identifying Extracellular Protein Interactions Using a Secreted Protein Microarray Platform

Technologies for identifying protein-protein interactions are largely biased towards intracellular binding partners. To address extracellular protein interactions, we have developed a secreted-protein microarray and validated its use as a fast and robust method for identifying receptor-ligand interactions. A set of eighty-nine immunoglobulin-domain receptors was screened and used to establish a scoring and specificity algorithm that revealed top hits as known binding partners and several new interactions for functional validation.